Additional file 2: of ZIKV infection activates the IRE1-XBP1 and ATF6 pathways of unfolded protein response in neural cells

The model of ZIKV infection in human neural cells, CCF-STTG1, and SK-N-SH cell lines. a, b CCF-STTG1 and SK-N-SH were infected with ZIKV at an MOI of 5 PFU/cell. RNA was extracted and first strand cDNA was synthesized. A standard curve of RT-qPCR was built and the absolute quantification of the viral nucleic acid was calculated respectively. Data represented three independent experiments and error bars indicate mean ± SD. c, d Detection of viral envelope protein at 24 hpi by confocal immunofluorescence microscopy. The nucleus was visualized using Hoechst 33258 (blue), and the envelope protein was stained with the anti-ZIKV envelope protein antibody followed by Goat anti-Mouse IgG secondary antibody, Texas Red®-X (red). All the images were captured at × 10 magnification. One out of three independent experiments is shown, (c) CCF-STTG1 and (d) SK-N-SH. (EPS 1006 kb)