Additional file 2: of Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1

Figure S1. Log transformed virulence factor RNA transcript expression in baseline cultures analyzed by grouping strains according to LRV-1 status compared by Mann-Whitney for the following targets: pooled expression (A), cpb (B), gp63 (C), mpi (D), hsp23 (E), hsp70 (F), hsp90 (G) and hsp100 (H). Figure S2. Log transformed virulence factor RNA transcript expression in supernatants post-macrophage infectivity at 24 and 48 h compared by LRV-1 status using t-test for the following targets: cpb-24 h (A), mpi-24 h (B), hsp70–24 h (C), pooled-48 h (D), cpb-48 h (E), and mpi-48 h (F). Figure S3. Log transformed virulence factor RNA transcript expression in baseline cultures analyzed by grouping strains according to species (L. V. panamensis versus other) compared by Mann-Whitney for the following targets: pooled expression (A), cpb (B), gp63 (C), mpi (D), hsp23 (E), hsp70 (F), hsp90 (G) and hsp100 (H). Figure S4. Log transformed virulence factor RNA transcript expression in supernatants post-macrophage infectivity at 24 and 48 h compared by species using t-test for the following targets: pooled VF-24 h (A), cpb-24 h (B), pooled VF-48 h (C), and cpb-48 h (D). Figure S5. Log transformed virulence factor RNA transcript expression in baseline cultures analyzed by grouping strains according to source of cultured isolate (ATCC® versus clinical) compared by Mann-Whitney for the following targets: pooled expression (A), cpb (B), gp63 (C), mpi (D), hsp23 (E), hsp70 (F), hsp90 (G) and hsp100 (H). Figure S6. Log transformed virulence factor RNA transcript expression in supernatants post-macrophage infectivity at 24 and 48 h compared by source of cultured isolate using t-test for the following targets: pooled VF-24 h (A), pooled VF-48 h (B), cpb-48 h (C), and mpi-48 h (D). (DOCX 1229 kb)