Additional file 2: of Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union

Figure S2. Immunohistochemistry of grafted hypocotyl sections – rhamnogalacturonan I (LM5, LM6, LM13, and LM16 epitopes). A and B – epitope detected abundantly in walls of graft union cells (arrows) and in low amount in walls of cortical cells (full arrow), epitope absent from extracellular material on the surface of graft union (arrowheads). A′ A, Calcofluor White. C and D – epitope present in cellular compartments of graft union cells (arrows), no epitope observed in extracellular material on the surface of graft union (arrowheads). C′ C, Calcofluor White. E – epitope detected in walls of some graft union cells (arrows), apart from extracellular material on the surface of graft union (arrowhead). E′ E, Calcofluor White. F – strong fluorescence signal in cell wall of sieve tubes (arrows). G – epitope absent from graft union cells (arrows) and from extracellular material (arrowheads). G′ G, Calcofluor White. c Calcofluor White. Scale bars: A, A′, C, C′, E, E′, G, and G′ = 50 μm; B, D, and F = 10 μm. (JPG 2868 kb)