Additional file 2: of The retinal environment induces microglia-like properties in recruited myeloid cells

Figure S2 Analysis of retinas for local progenitors following ONC. (A) Transient appearance of CD34+ cells in the retinas of non-ablated, non-chimeric CD11cDTR/GFP mice after ONC. CD34+ cell numbers (mean ± SD, n = 4) were determined by direct counts using fluorescence microscopy. (B, C) Immunofluorescence staining showing CD34+ cells in the ganglion cell layer of the retina at 4 days post-ONC. (D) Analysis of bone marrow cells for CD34 expression. (E) Analysis of a non-chimeric B6 mouse 4 days post-ONC revealed the occasional group of CD117+ cells in the inner plexiform layer. (F) Analysis of fetal liver cells for CD117 expression. (DOCX 1308 kb)