Additional file 2: of The piRNA pathway responds to environmental signals to establish intergenerational adaptation to stress

Figure S2. piRNA production in different species and in the pmk-1 mutant. (A) Log2 ratio of the count of 21U small RNA in P. pacificus between 25 °C and 20 °C. piRNAs, identified by the presence of a conserved upstream motif, are reduced relative to all other 21U-RNAs. 4956 such loci were detected and used for analysis. (B) Difference in motif-dependent piRNA precursors at 25 °C and 20 °C in P. pacificus. (C) Ratio of the amount of motif-dependent piRNAs at 20 °C over 25 °C in 4 different strains of C. briggsae. AF16 is the standard laboratory strain. In red are tropical strains and in blue are temperate strains. 2431 such piRNA loci were detected in the reference strain AF16 and used for analysis. (D) Log2 reads per million of motif-dependent piRNAs in the wild-type (WT) strain and in the pmk-1 mutant at 20 °C and 25 °C. (E) Change in prg-1 mRNA level between 20 °C and 25 °C. (PDF 536 kb)