Additional file 2: of Stationary phase persister formation in Escherichia coli can be suppressed by piperacillin and PBP3 inhibition

Figure S2. Carbenicillin and ciprofloxacin persister assays. Cell cultures were treated with 200 µg/mL piperacillin (PIP) at t= 4 h. Cells in control culture were treated with an equal volume of water (-). At 24 h, cultures were washed to remove chemicals and diluted in fresh LB containing 200 µg/mL carbenicillin (CAR) or 1 µg/mL ciprofloxacin (CIP). Survival fractions (A and C) were monitored at the indicated time points. CFU/mL are provided (B and D). * p< 0.05 (t- test). Data represent at least three biological replicates. Each data point was denoted as mean ± s.e. (TIF 165 kb)