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Additional file 2: of Secretome of adipose-derived mesenchymal stem cells promotes skeletal muscle regeneration through synergistic action of extracellular vesicle cargo and soluble proteins

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posted on 05.04.2019, 05:00 by Robert Mitchell, Ben Mellows, Jonathan Sheard, Manuela Antonioli, Oliver Kretz, David Chambers, Marie-Theres Zeuner, James Tomkins, Bernd Denecke, Luca Musante, Barbara Joch, Florence Debacq-Chainiaux, Harry Holthofer, Steve Ray, Tobias Huber, Joern Dengjel, Paolo Coppi, Darius Widera, Ketan Patel
Figure S2. (A) The secretome generated from fibroblast cells under identical conditions to ADSC contains a significantly lower amount of total protein. (B-C) Characterising the fibroblast EVs using NTA found a significantly lower concentration per mL, with a similar size range to ADSC EVs (< 500 nm). (D-F) Investigating the functional characteristics of the fibroblast secretome found no significant increase in cellular proliferation compared to PBS control (D). There was no change from control levels of C2C12 cell fusion with treatment with fibroblast secretome (E). Fibroblast secretome demonstrated a slight non-significant increase in percentage gap closure on the in vitro wound assay (F). p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***). Three batches of fibroblast secretome were generated and tested. (JPG 243 kb)

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