Additional file 2: of Reproducible changes in the gut microbiome suggest a shift in microbial and host metabolism during spaceflight

Figure S1. Receiver operating characteristic (ROC) curves showing the performance of STARMAPs using simulated data. In each panel, STARMAPs was applied to 2000 simulated pairs of datasets that either shared a set of differentially abundant species (true positives) or involved distinct sets of differentially abundant species (true negatives), and the sensitivity (true positive fraction) and specificity (1 – false positive fraction) of STARMAPs were plotted at given STARMAPs omnibus P values ranging from 0 to 1. The enlarged point on each curve indicates the sensitivity and specificity estimated at omnibus P = 0.05. Simulations were done with varying amplitudes of differential abundance [i.e., effect size in log2(FC); rows] over a range of parameters representing different scenarios (columns). These varying parameters included the sample size (N) for each group, the variance (s) of the log2(FC) applied to the second dataset of a given pair of datasets, and the proportion of species commonly found in a given pair of datasets (overlap). (PDF 2000 kb)