Additional file 2: of Redirecting differentiation of mammary progenitor cells by 3D bioprinted sweat gland microenvironment

Figure S2. Characteristics of three-dimensional (3D) bioprinted sweat gland (SG) microenvironment. (a) The content of DNA of centrifuged and Non-centrifuged mouse SG-extracellular matrix (ECM) proteins measured by spectrophotometer. The control was phosphate-buffered saline (PBS) which had no DNA (n = 3). The result demonstrated that there were no cells in the dermal homogenates. In the statistical analysis, one-way ANOVA was used to measure the difference between these three groups. In each group comparison, SNK-q test was used. *p < 0.05, **p < 0.01. (b) The process of 3D bioprinting with bioprinter. (c) The porous structure of 3D bioprinted SG microenvironment was observed using scanning electron microscopy (SEM) (scale bar, 100 μm). (d) Cell viability of the 3D bioprinted SG microenvironment. The live cells were labeled with Calcein AM and dead cells with EthD-1 (scale bar, 500 μm). (e) Cell morphology in groups of SG-ECM, Non-bioprinted and Non-protein at different time points (scale bar, 50 μm, 200 μm). (JPG 101 kb)