Additional file 2: of Preemptive intrathecal administration of endomorphins relieves inflammatory pain in male mice via inhibition of p38 MAPK signaling and regulation of inflammatory cytokines

Figure S1. Phosphorylated p38 MAPK and the mu-opioid receptor were expressed in mouse ipsilateral L4 DRG neurons. (A) Double immunostaining of MOR (green) and NeuN (red) was investigated in L4 DRG tissues of control group and saline-treated CFA group. (B) Double immunostaining of P-p38 MAPK (red) and NeuN (green) was conducted in L4 DRG tissues of control group and saline-treated CFA group. Arrows indicate co-expression. n = 4 animals/group, > 500 neurons per animal were assessed. Scale bar = 50 μm. (PPTX 2054 kb)