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Additional file 2: of Neoadjuvant neratinib promotes ferroptosis and inhibits brain metastasis in a novel syngeneic model of spontaneous HER2+ve breast cancer metastasis

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posted on 2019-08-14, 03:59 authored by Aadya Nagpal, Richard Redvers, Xiawei Ling, Scott Ayton, Miriam Fuentes, Elnaz Tavancheh, Irmina Diala, Alshad Lalani, Sherene Loi, Steven David, Robin Anderson, Yvonne Smith, Delphine Merino, Delphine Denoyer, Normand Pouliot
Figure S2. Principal component analysis of neratinib-treated versus untreated TBCP-1 cells and ferroptotic/apoptotic response to inhibitors. (A) Sub-confluent cultures of TBCP-1 cells were treated for 24 h with vehicle (DMSO) or neratinib (300 nM). Cell viability under those conditions was analysed by flow cytometry. Gating for all events (P1), single cells (P2) and viability (P3) is shown in the top panels and overall viability in control and neratinib-treated cultures, and changes in cell morphology (rounding) induced by neratinib are shown in the bottom panels. (B) Principal component analysis of neratinib-treated versus untreated TBCP-1 cells. Control and neratinib-treated cell lysates were subjected to RNA isolation and sequencing as described in the “Methods” section. (C) Representative images of TBCP-1 cell death induced by neratinib or BH3 mimetics and rescue by ferroptosis or apoptosis inhibitors. Arrows show extensive blebbing induced by BH3 mimetics. Scale bar = 50 μm. (TIF 22771 kb)

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