Additional file 2: of N-acetylcysteine decreases malignant characteristics of glioblastoma cells by inhibiting Notch2 signaling

Figure S2. NAC decreases mRNA levels of Hes1 and Hey1. A and B, The mRNA analysis of Hes1 (A) and Hey1 (B) following dose-dependent treatment of NAC. Cells were treated with NAC (5, 10 or 20 mM) for 24 h. C and D, The mRNA analysis of Hes1 (C) and Hey1 (D) following time-dependent treatment of NAC. Cells were treated with NAC (10 mM) for 6, 12 or 24 h. β-actin was used as a housekeeping gene. E and F, The western blot analysis of Notch2, Notch3 using Scramble, si-Notch2 or si-Notch3 in U87 (E) and U251 (F) cells. β-actin was used as a loading control. All data are presented as means ± SD of three independent experiments. * P < 0.05 compared with control group or Scramble group. (TIF 6153 kb)