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Additional file 2: of Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection
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posted on 2019-07-30, 04:18 authored by Jérôme Caron, Véronique Pène, Laia Tolosa, Maxime Villaret, Eléanor Luce, Angélique Fourrier, Jean-Marie Heslan, Samir Saheb, Eric Bruckert, María Gómez-Lechón, Tuan Nguyen, Arielle Rosenberg, Anne Weber, Anne Dubart-KupperschmittFigure S2. Confirmation of the correct homologous recombination during the insertion of the therapeutic cassette. (A-B) Forward (A) and reverse (B) sequence alignments for the 5′ homology arm between corr-FH-iPSC (lower lane) and the plasmid used for the homologous recombination (upper lane). (C-D) Forward (C) and reverse (D) sequence alignments for the 3′ homology arm between corr-FH-iPSC (lower lane) and the plasmid used for the homologous recombination (upper lane) . (TIF 3705 kb)