41021_2019_129_MOESM2_ESM.docx (205.57 kB)
Additional file 2: of Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy
journal contribution
posted on 2019-06-05, 05:00 authored by Takenori Ishida, Nobutoshi Fujihara, Tomoki Nishimura, Hisakage Funabashi, Ryuichi Hirota, Takeshi Ikeda, Akio KurodaFigure S1. Asbestos-binding proteins in RAW 264.7 cells. RAW 264.7 cell lysate was incubated with asbestos. Proteins bound to asbestos were subsequently separated via 12.5% SDS-polyacrylamide gel electrophoresis. Protein bands were excised from the gel, digested with sequencing-grade trypsin, and analyzed via liquid chromatography-tandem mass spectrometry. The peptide fingerprints obtained were used in protein searches conducted using Mascot. The proteins identified are shown on the right. (DOCX 205 kb)