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Additional file 2: of Lack of hepatic apoE does not influence early Aβ deposition: observations from a new APOE knock-in model

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posted on 2019-10-18, 09:28 authored by Tien-Phat Huynh, Chao Wang, Ainsley Tran, G. Tabor, Thomas Mahan, Caroline Francis, Mary Finn, Rebecca Spellman, Melissa Manis, Rudolph Tanzi, Jason Ulrich, David Holtzman
Figure S2. ApoE isoforms differentially influence Aβ plaque deposition in APP/PS1/APOE-TR mice. a Brain sections from 3-month-old APP/PS1/APOE-TR mice were immunostained with anti-Aβ antibody 3D6 and the extent of Aβ deposition in the cortex quantified. Two-way ANOVA analysis found a significant effect of apoE isoform (F2,38 = 10.13, p = 0.0003) but no significant effect of sex (F1,38 = 0.001177, p = 0.9728), and no interaction (F2,38 = 0.06101, p = 0.9409). Post hoc analysis comparing apoE isoform within each sex found a statistically significant increase in Aβ deposition in male apoE2- (p = 0.0010) and apoE4-expressing (p = 0.0430) mice compared to apoE3, APP/PS1/E2 = 8 males, 9 female; APP/PS1/E3 = 11 males, 4 females; APP/PS1/E4 = 7 males, 5 females.b Since there was no effect of sex on Aβ staining, data for both males and females were pooled together. A one-way ANOVA was performed (F = 11.92, p < 0.0001), followed by Tukey’s post hoc test for multiple comparisons. c Brain sections from 4-month-old APP/PS1/APOE-TR mice were stained with X-34 dye that recognizes only fibrillar plaques and the cortical area stained by X-34 was quantified. There was a significant effect of genotype (F2,37 = 8.701, p = 0.0008) but no significant effect of sex (F1,37 = 0.6014, p = 0.4430), and no interaction (F2,37 = 0.2230, p = 0.8012). Post hoc analysis comparing apoE isoform within each sex found a statistically significant increase in X-34 staining in male apoE2-expressing mice compared to apoE3 (p = 0.0011), APP/PS1/E2 = 8 males, 9 female; APP/PS1/E3 = 10 males, 4 females; APP/PS1/E4 = 7 males, 5 females. d Since there was no effect of sex on X-34 staining, data for both males and females were pooled together. A one-way ANOVA was performed (F = 11.69, p = 0.0001), followed by Tukey’s post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, **** p < 0.0001. All values are reported as mean ± SEM. (TIF 605 kb)

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