Additional file 2: of Involvement of a dihydrodipicolinate synthase gene (FaDHDPS1) in fungal development, pathogenesis and stress responses in Fusarium asiaticum

Figure S1. Generation and identification of Fusarium asiaticum FaDHDPS1 gene deletion mutants. (A) Gene replacement and complementation strategy for FaDHDPS1. The gene replacement cassette HPH-HSV-tk contains the hygromycin resistance gene and the herpes simplex virus thymidine kinase gene. Primer binding sites are indicated by arrows (see Additional file 1: Table S1 for the primer sequences). (B) Southern blot hybridization analysis of 2021, ΔFaDHDPS1 and ΔFaDHDPS1-C using the upstream fragment of FsDHDPS1 as a probe, and genomic DNA were digested with SphI. (TIF 1328 kb)