Additional file 2: of Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis

Figure S1. circFNDC3B inhibits proliferation of BC cells. a and b qRT-PCR analysis for circFNDC3B and FNDC3B mRNA in UM-UC-3 cells treated with siRNAs or T24 cells transfected with circFNDC3B overexpression vector. c and d The effect of si-circFNDC3B on cell proliferation of UM-UC-3 cells was assessed by MTS and colony formation assays. e and f Assessment of cell proliferation of T24 cells transfected with circFNDC3B overexpression vector by MTS and colony formation assays. Data indicate means±SEM of three experiments. **P < 0.01 (Student’s t-test). Figure S2. circFNDC3B inhibits migration and invasion of BC cells. a and b The cell migratory and invasive capabilities were examined in UM-UC-3 cells treated with circFNDC3B siRNAs using wound healing assay, transwell migration and Matrigel invasion assays. c and d The cell migratory and invasive abilities were assessed after T24 cells were transfected with circFNDC3B overexpression vector. a and c, scale bar,200 μm; b and d, scale bar, 100 μm. Data indicate means±SEM of three experiments. *P < 0.05, **P < 0.01 (Student’s t-test). Figure S3. The identification and confirmation of circFNDC3B-related downstream molecules in BC cells. a The sequence alignment of miR-1178-3p with circFNDC3B. The mutant bases are depicted in red. b and c qRT-PCR analysis of 8 cancer-related genes after transfection with circFNDC3B siRNAs in T24 and UM-UC-3 cells. d The sequence alignment of miR-1178-3p with 3’UTR of p21 (predicted by Targetscan). e The sequence alignment of miR-1178-3p with 5’UTR of G3BP2. The mutant bases are depicted in red. Data indicate means±SEM of three experiments. *P < 0.05, **P < 0.01 (Student’s t-test). (PDF 13185 kb)