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Additional file 2: of Inhibition of mitochondrial respiration prevents BRAF-mutant melanoma brain metastasis

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posted on 2019-04-10, 05:00 authored by Terje Sundstrøm, Lars Prestegarden, Francisco Azuaje, Synnøve Aasen, Gro Røsland, Jobin Varughese, Marzieh Bahador, Simon Bernatz, Yannick Braun, Patrick Harter, Kai Skaftnesmo, Elizabeth Ingham, Lisa Mahakian, Sarah Tam, Clifford Tepper, Kjell Petersen, Katherine Ferrara, Karl Tronstad, Morten Lund-Johansen, Rudi Beschorner, Rolf Bjerkvig, Frits Thorsen
Figure S2. Generation of organ samples for RNA sequencing, brain metastasis gene signature and Connectivity Map analysis. a BLI five weeks after intracardiac injection of 5 × 105 H1_DL2 cells in NOD.CB17-Prkdcscid/NcrCrl mice (n = 7). M1-M7, indicate mouse 1 to 7. b Fluorescence-activated cell sorting (FACS) of GFP-positive tumor cells from the metastatic lesions. The sorted cells were analyzed by RNA-seq (n = 7). c Heatmap of the 108-gene brain metastasis signature with organ samples in rows and genes in columns. d The brain metastasis gene signature with the left two panels showing 54 upregulated genes in brain metastases and the right two panels showing 54 downregulated genes. e Query results from the Connectivity Map (cMap) database using the 108-gene signature. A score of < 0 to − 1 means net reversal of the signature (negative correlation) and a score of > 0 to + 1 means net induction of the signature (positive correlation). (TIF 2033 kb)

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