Additional file 2 of Impact of lignans in oilseed mix on gut microbiome composition and enterolignan production in younger healthy and premenopausal women: an in vitro pilot study

Additional file 2: Fig. S2. Weighted UniFrac-based Principal Coordinates Analysis (PCoA) of the faecal-derived microbial communities of premenopausal and younger healthy women over 24 h of fermentation in the presence of oilseed mix, inulin or without additions. Left, PCoA plot showing all fermentation samples, coloured by group of women (premenopausal, light blue vs. younger healthy, orange). A significant separation between groups was found, regardless of experimental condition (oilseed mix, inulin and negative control—“Ctr-”) and time point (T0, T5 and T24) (p value < 1 × 10−4, permutation test with pseudo-F ratio). Right, PCoA plots showing the fermentation samples for premenopausal women (top panel) and younger healthy women (bottom panel). Within each group of women, the samples separate significantly by experimental condition (oilseed mix, green; inulin, red; Ctr-, yellow) and time point (T0, circle; T5, square; T24, diamond) (p value < 0.001).