Additional file 2 of High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers

Additional file 2 : Suppl. Figure 2: Systematic view of protein changes between slow and fast muscle fibers. A) Principal component analysis (PCA) revealed a clear separation of type I and type IIa fibers from the soleus muscle and B) indicates the main components responsible for the separation (green for type IIa and blue for type I). C) Similarly, PCA clearly confirmed the separation of protein intensities for type IIb fibers from the EDL, TA, and Gast. D) Blue labeled circles represent the main components responsible for the separation. E) Overview of co-immunostaining of soleus cross-sections with antibodies that recognize TMEM65 and the mitochondrial outer membrane protein TOM20. F) Co-immunostaining of soleus cross-sections with TMEM65 and antibodies that bind specifically to slow MYH7 (type) and fast MYH2 (type IIa) isoforms. G) Quantitative analysis of TMEM65 fluorescence signal intensities in different fiber types in the soleus muscle. Significance was tested by two-sided t-tests (n = 297). H) EDL cross-sections immunostained with TMEM65, MYH2, and MYH4. I) Quantitative analysis of the TMEM65 fluorescence signal in EDL myofibers. Significances were tested by two-sided t-tests (n = 200)