Additional file 2: of Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders

Avidity index of pooled serums from Group 1 and Group 2, and neutralizing capacity of serums against hemolytic activity of venom of L. laeta. (A) Comparison of avidity index of pooled serums of Group 1 and Group 2 (1:100 diluted), treated with 6 M urea by IgG avidity ELISA. (ns) indicates not statistically significant. (B) Human erythrocytes were sensitized for 1 h at 37 °C with 25 μg/mL of venom of L. laeta in the presence or absence of pooled sera of Group 1 or Group 2 at 1:1, 1:10 and 1:100 dilutions, and evaluated in a complement-dependent hemolysis assay. Negative control was incubated only with VBS and with not presence of complement serum (control without complement). Results were expressed as percentage of hemolysis. The assays were made in duplicate for a total of two independent experiments and results are expressed as mean ± SEM. Significance was evaluated with an ANOVA one-way with Bonferroni post-hoc test; (ns) indicates not statistically significant. (TIF 9838 kb)