Additional file 2: of Genomic variation and biogeography of Antarctic haloarchaea

Figures S1-S8 and Tables S1-S13: Figure S1. Archaellin protein sequence alignment. Figure S2. arCOG functional classes of genes present on ACAM34 and R1S1 secondary replicons. Figure S3. arCOG functional classes of the genes within HIRs specific to R1S1 and DL1, DL31 and Hht. litchfieldiae tADL. Figure S4. New HIRs present in R1S1 that are shared with Hht. litchfieldiae tADL. Figure S5 Genomic islands on primary replicons of Antarctic haloarchaea. Figure S6. Metagenome coverage and HCA of selected secondary replicons. Figure S7. Contigs assembled from metagenomes mapped to replicons. Figure S8. Clustering and scaling of samples. Table S1. Description of the Rauer Islands and Vestfold Hills hypersaline lakes sampled in this study. Table S2. Antarctic lake metagenomes used in this study. Table S3. Unique transposases of ACAM34 and R1S1. Table S4. Unique protein-coding genes. Table S5. Unique sequence duplications and non-coding RNAs on the R1S1 primary replicon. Table S6. Regions with low sequence similarity between ACAM34 and R1S1 primary replicons. Table S7. New HIRs identified in R1S1. Table S8. HIR conserved between Hrr. lacusprofundi R1S1 and DL1 that encodes a type I-B CRISPR system. Table S9. R1S1 CRISPR spacer matching Hlac-Pro1 in ACAM34. Table S10. Presence of 16S rRNA gene sequences for known Antarctic haloarchaeal species in Antarctic hypersaline lakes. Table S11. Relative abundance of Hrr. lacusprofundi ACAM34, Hht. litchfieldiae tADL, DL31 and DL1 in Antarctic lake metagenomes. Table S12. Relative abundance of lake taxa assessed from read coverage and taxonomic assignment of contigs assembled from metagenome data. Table S13. Genome coverage and percent identity for contigs mapped to replicons of Hrr. lacusprofundi R1S1 and ACAM34, Hht. litchfieldiae tADL, DL31 and DL1. (PDF 7786 kb)