Additional file 2: of Genistein inhibits stemness of SKOV3 cells induced by macrophages co-cultured with ovarian cancer stem-like cells through IL-8/STAT3 axis

Figure S2. GEN alleviated stemness of SKOV3 cells induced by Co-CM. OVCAR-3 cells treated with Co-CM from the co-culture of OVCAR-3-derived OCSLCs with THP-1 macrophages and were treated with or without different concentrations of GEN (10, 20, and 40 μM). The sphere and colony formation rate (A and B, scale bar, 100 μm) and expression levels of CD133 and CD44 (C) as well as Nanog and Oct4 (D) in OVCAR-3 cells were shown.*P < 0.05, vs OVCAR-3 cells induced by co-culture were treated with vehicle (0.1% DMSO). #P < 0.05, vs OVCAR-3 cells induced by co-culture treated with GEN (10.0 μM). These experiments were performed in triplicate. (TIF 24203 kb)