Additional file 2: of Functional and mechanistic studies reveal MAGEA3 as a pro-survival factor in pancreatic cancer cells

2019-07-08T05:00:00Z (GMT) by Biswajit Das Shantibhusan Senapati
Figure S1. Isolation and characterisation of mouse pancreatic epithelial cells. a Bright field micrograph of mouse pancreatic epithelial cells along with fibroblast cells on collagen coated culture plate after 24 h of isolation and seeding. Scale bar = 200 μm. b Bright field micrograph showing the reduced fibroblast cells (differentiated completely and stopped dividing) and attached epithelial cells on collagen coated culture plate after 5 days of 1st split. Scale bar =200 μm. c Bright field micrograph showing homogeneous population of mouse pancreatic epithelial cells. Scale bar =200 μm. d Immunofluorescence staining confirms the isolated cells are of epithelial origin. E-cadherin is used as epithelial marker. Images captured with 40X objective, scale bar = 20 μm. e Bright field micrograph showing no gross change in morphology after overexpressing the mouseKRASG12D or MAGEA3 or MAGEA3-HA or GFP proteins in the isolated mouse pancreatic epithelial cells (stable cells, selected with puromycin at a concentration of 3 μg/mL). Scale bar = 200 μm. f Immunoblot showing overexpression of mouseKRASG12D or huMAGEA3 or huMAGEA3-HA in mouse primary pancreatic epithelial stable cells. (JPG 2462 kb)