Additional file 2: of FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma

Figure S2. Co-effects of FUS and circ_002136 on the expression of downstream molecules and angiogenesis. (A) The expression of miR-138-5p was co-regulated by both FUS and circ_002136. Values represent the means ± SD (n = 5, each group). **P < 0.01 vs. FUS(−)NC + circ_002136(−)NC group. ##P < 0.01 vs. FUS (−) + circ_002136(−)NC group. (B-D) The co-effects of FUS and circ_002136 on the mRNA and protein expression levels of SOX13 and SPON2 in GECs were evaluated by qRT-PCR and western blot. Data are presented as the means ± SD (n = 3, each group). **P < 0.01 vs. FUS(−)NC + circ_002136(−)NC group. ##P < 0.01 vs. FUS(−) + circ_002136(−)NC group. (E) The co-effects of FUS and circ_002136 on the viability of GECs were evaluated by the CCK-8 assay. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs. FUS(−)NC + circ_002136(−)NC group. ##P < 0.01 vs. FUS(−) + circ_002136(−)NC group. (F) The co-effects of FUS and circ_002136 on the migration of GECs were evaluated by the transwell assay. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs. FUS(−)NC + circ_002136(−)NC group. ##P < 0.01 vs. FUS(−) + circ_002136(−)NC group. The scale bar represents 100 μm. (G) The co-effects of FUS and circ_002136 on the tube formation of GECs were evaluated by the Matrigel tube formation assay. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs. FUS(−)NC + circ_002136(−)NC group. ##P < 0.01 vs. FUS(−) + circ_002136(−)NC group. The scale bar represents 100 μm. (TIF 1639 kb)