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13287_2017_658_MOESM2_ESM.mov (15.77 MB)

Additional file 2: of Direct conversion of human fibroblasts to functional excitatory cortical neurons integrating into human neural networks

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posted on 2017-09-29, 05:00 authored by Giedre Miskinyte, Karthikeyan Devaraju, Marita Grønning Hansen, Emanuela Monni, Daniel Tornero, Niels Woods, Johan Bengzon, Henrik Ahlenius, Olle Lindvall, Zaal Kokaia
Three-dimensional video reconstruction of iCtx cell ex vivo transplanted onto adult human-derived cortical tissue. Fly-through animation of three-dimensional data illustrating pyramidal shape of iCtx cell (in red) with elaborated neurites extending throughout the whole depth of the adult human cortical slice at 3 weeks after ex vivo transplantation. Note many spines of different size and shape including mushroom-like ones. Many Hoechst-stained cell nuclei of human cortex (in blue) are distributed throughout the slice. Scale bar in left bottom corner. (MOV 16146 kb)

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