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Additional file 2: of Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells

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posted on 2018-03-09, 05:00 authored by Ruhang Tang, Liufang Jing, Vincent Willard, Chia-lung Wu, Farshid Guilak, Jun Chen, Lori Setton
Figure S1. Showing immunofluorescence of sorted T-GFP+ cells promoted to differentiate in culture. (A) Immunocytofluorescence showed the positive expression of CD24 (green) and (B) positive expression of CD239 (red) in sorted T-GFP+ cells when differentiated in monolayer for 12 days using step 1b and step 2 of NPDM protocol. (C) Yellow denotes colocalization of the markers. All cells counterstained with DAPI. Scale bar = 20 Îźm. (DOCX 995 kb)

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National Institute of Arthritis and Musculoskeletal and Skin Diseases

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    Stem Cell Research & Therapy

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    Human induced pluripotent stem cellsIntervertebral discDegenerative disc diseaseNucleus pulposusDirected differentiationChondrocyteCartilage

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