Additional file 2: of Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells

Figure S1. Showing immunofluorescence of sorted T-GFP+ cells promoted to differentiate in culture. (A) Immunocytofluorescence showed the positive expression of CD24 (green) and (B) positive expression of CD239 (red) in sorted T-GFP+ cells when differentiated in monolayer for 12 days using step 1b and step 2 of NPDM protocol. (C) Yellow denotes colocalization of the markers. All cells counterstained with DAPI. Scale bar = 20 Îźm. (DOCX 995 kb)