Additional file 2: of Determinant roles of dendritic cell-expressed Notch Delta-like and Jagged ligands on anti-tumor T cell immunity

Figure S2. Clustered DLL1 and soluble JAG1 constructs decrease the expression of checkpoint molecule PD-1 on T-effector memory cells in vitro. Purified T cells were stimulated in vitro in a T:DC (3:1) stimulation co-culture with allogenic dendritic cells in the presence of CD3/CD28 beads (1 μg/mL) for four days with or without treatment with the indicated concentrations of clustered DLL1 or monovalent soluble JAG1 constructs. Expression of CTLA-4 and PD-1 was assessed on gated populations as indicated by flow cytometry. Dot plots from a representative experiment out of two independent experiments with duplicates are shown. (PPTX 8407 kb)