Additional file 2: of Chondrogenic induction of human osteoarthritic cartilage-derived mesenchymal stem cells activates mineralization and hypertrophic and osteogenic gene expression through a mechanomiR

Figure S2. Western blot densitometric analysis of chondrogenic markers and hypertrophic markers in undifferentiated OASC2 upon transfection of miR-365 or its inhibitor. To quantify the protein expression level of MMP13 (a), COL10A1 (b), ADAMTS-5 (c), SOX9 (d) and RUNX2 (e) in undifferentiated OASC2 transfected with miR-365 mimic or miR-365 inhibitor, densitometric analysis of the western blots in terms of gray intensity was performed using ImageJ for at least three experimental repeats. The densitometric intensity was normalized to that of β-ACTIN which served as the loading control. The average of the mimic control group was normalized to 1 fold. Data are presented as mean ± SD for each group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, *****p < 0.00001, *********p < 0.000000001. (JPG 1504 kb)