Additional file 2: of An amplicon-based sequencing framework for accurately measuring intrahost virus diversity using PrimalSeq and iVar

iSNV frequencies calculated using pseudo replicates (Figure S1.), sensitivity of measuring intrahost diversity at 5% (Figure S2.), metagenomic sequencing coverage depth (Figure S3.), and validation of iVar for intrahost single-nucleotide variant calling (Figure S4.), consensus calling (Figure S5.), and trimming (Figure S6.). (PDF 7109 kb)