13059_2018_1618_MOESM2_ESM.pdf (6.94 MB)
Additional file 2: of An amplicon-based sequencing framework for accurately measuring intrahost virus diversity using PrimalSeq and iVar
journal contribution
posted on 2019-01-08, 05:00 authored by Nathan Grubaugh, Karthik Gangavarapu, Joshua Quick, Nathaniel Matteson, Jaqueline Jesus, Bradley Main, Amanda Tan, Lauren Paul, Doug Brackney, Saran Grewal, Nikos Gurfield, Koen Van Rompay, Sharon Isern, Scott Michael, Lark Coffey, Nicholas Loman, Kristian AnderseniSNV frequencies calculated using pseudo replicates (Figure S1.), sensitivity of measuring intrahost diversity at 5% (Figure S2.), metagenomic sequencing coverage depth (Figure S3.), and validation of iVar for intrahost single-nucleotide variant calling (Figure S4.), consensus calling (Figure S5.), and trimming (Figure S6.). (PDF 7109 kb)
