Additional file 2 of A pipeline for targeted metagenomics of environmental bacteria

Additional file 2: Figure S2. The background fluorescence in the green channel of flow cytometric measurements was set to 10 RU (blue line), based on comparisons betwen EUB-338 and Non-338 probes hybridized to 1% formaldehyde fixed Gramella forsetii (A, B) and Micrococcus sp. (C, D) samples. The Non-338 control of Gramella forsetii (A) was not stained with DAPI, the Non-338 control of Micrococcus sp. (C) was stained with DAPI. Green fluorescence was detected with a 530/40 nm filter, blue fluorescence with a 460/50 nm filter. 5000 events were recorded for Gramella forsetii (A, B) and 2000 for Micrococcus sp. (C, D).