Additional file 2: of A chicken bioreactor for efficient production of functional cytokines

Figure S2. Interferon α2A mono-dispersity, size and purity analysis. A) Size-exclusion chromatography multi-angled light scattering (SEC-MALS) was used to determine the molecular and mono-dispersity of Interferon α2A in solution. Interferon α2A eluted from a Superdex-200 Increase 10/300 GL size exclusion column as a single peak with slight trailing edge (≥ 96% purity; As > 2) with apparent molecular mass of ~ 33.2 ± 2 kDa and an Rs of 2.43 ± 0.2 nm (mean ± SD, n = 3). The molecular mass average across the elution profile is 18.7 kDa with average mono-dispersity (Mw/Mn = 1.038) and no significant aggregation. B) Elution profile of Interferon α2A from an RPC 5 4.6/150 ST column run. Chromatogram shows single sharp peak, with ≥98% purity. (DOCX 486 kb)