Additional file 2: Figure S3. of Adipose tissue-derived stem cells suppress hypertrophic scar fibrosis via the p38/MAPK signaling pathway

The effect of ADSC-CM on the primary NS-derived fibroblasts in vitro. (A-C) qRT-PCR analysis for the mRNA levels of Col1, Col3, and α-SMA in NSFs stimulated with different concentrations of ADSC-CM (10 %, 20 %, 40 %, 80 %) for 24 h; graph representeds the expression of Col1, Col3, and α-SMA relative to that of GAPDH. (D–G) Immunoblot analysis for the protein expression of Col1, Col3, and α-SMA in NSFs stimulated with different concentrations of ADSC-CM (10 %, 20 %, 40 %, 80 %) for 24 h; graph shows the relative band density to β-actin. (H, I) Immunoblot analysis for the expression of p-p38 stimulated for 1 h with different concentrations of ADSC-CM (10 %, 20 %, 40 %, 80 %). Every experiment was repeated three times at least. The data are shown as mean ± SEM from three independent experiments (*P < 0.05; **P < 0.01). (TIF 1346 kb)