Additional file 2: Figure S2. of Ziziphi spinosae lily powder suspension in the treatment of depression-like behaviors in rats

Chromatograms of 5-HIAA under optimal conditions. A: Chromatogram of the 5-HIAA standard. B: Chromatogram of the brain sample. The chromatographic separation for 5-HIAA was carried out using 2000 ml mobile phase consisting of 20.7 g NaH2PO4, 0.735 g Octanesulfonic Acid Sodium, 200 ml acetonitrile, 200ul triethylamine, 25umol EDTA at a flow rate of 1 ml/min (pH = 3.8). The mobile phase was filtered with 0.22um filter membrane and was degassed on line before measurement. The injection volume was 20 μl. (TIF 263 kb)