12974_2016_783_MOESM2_ESM.pptx (42.91 kB)
Additional file 2: Figure S2. of WWL70 attenuates PGE2 production derived from 2-arachidonoylglycerol in microglia by ABHD6-independent mechanism
presentation
posted on 2017-01-10, 05:00 authored by Mikiei Tanaka, Sean Moran, Jie Wen, Kwame Affram, Tinghua Chen, Aviva Symes, Yumin ZhangEffect of WWL70 in recombinant COX assay. Ovine recombinant COX-1 (A) or human recombinant COX-2 (B) was pre-incubated in 0.1 M Tris-HCl (pH 8.0) and EDTA (1 mM) buffer with WWL70 (10 μM), SC-560 (0.33 μM), or Dup-697 (0.3 μM) for 5 min at 27 °C. Then, 10 μM of AA was added and incubated for 1 min at 27 °C. The reaction mixture was applied for prostaglandins EIA to measure total amount of prostaglandins. (PPTX 42 kb)