13071_2016_1682_MOESM2_ESM.tif (807.5 kB)
Additional file 2: Figure S2. of Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene
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posted on 2016-07-19, 05:00 authored by Saúl Rojas-Sánchez, Elisa Figueroa-Angulo, Rodrigo Moreno-Campos, Luis Florencio-Martínez, Rebeca Manning-Cela, Santiago Martínez-CalvilloNorthern blot analysis of the tagged U2 snRNA. The experiment was performed with total RNA from cells transfected with construct 1 (pComp), construct 6 (pBS + 6/+12), the unrelated vector pLMRIB or mock-transfected cells. The probe was the oligonucleotide LmjU2tag-Rev, which specifically recognizes the tag sequence. (TIF 807 kb)