12943_2015_440_MOESM2_ESM.pptx (755.96 kB)
Additional file 2: Figure S2. of The polarity protein Scrib mediates epidermal development and exerts a tumor suppressive function during skin carcinogenesis
presentation
posted on 2015-09-17, 05:00 authored by Helen Pearson, Edwina McGlinn, Toby Phesse, Holger Schlüter, Anuratha Srikumar, Nathan Gödde, Christina Woelwer, Andrew Ryan, Wayne Phillips, Matthias Ernst, Pritinder Kaur, Patrick HumbertScrib is not essential for epidermal homeostasis in adult mice. (A) qRT-PCR to detect Lgr5 confirmed Scrib +/+, Scrib +/ fl and Scrib fl/fl adult dorsal epidermis display a similar level of Lgr5 mRNA transcript expression (P ≥ 0.1013, unpaired t-test, error bars = SD, n = 3). (B) IHC to detect cytokeratin 6 (K6) in Scrib +/+, Scrib +/ fl and Scrib fl/fl adult dorsal epidermis (scale bar = 50 μm, n = 3). (C) IF to detect cytokeratin 5 (K5, green), cytokeratin 10 (K10, red) and DAPI (blue) in Scrib+/+, Scrib +/ fl and Scrib fl/fl adult dorsal epidermis (scale bar = 50 μm, n = 3). Adult dorsal epidermis was harvested from mice 100 days old. (PPTX 755 kb)
