Additional file 2: Figure S2. of The polarity protein Scrib mediates epidermal development and exerts a tumor suppressive function during skin carcinogenesis

Scrib is not essential for epidermal homeostasis in adult mice. (A) qRT-PCR to detect Lgr5 confirmed Scrib +/+, Scrib +/ fl and Scrib fl/fl adult dorsal epidermis display a similar level of Lgr5 mRNA transcript expression (P ≥ 0.1013, unpaired t-test, error bars = SD, n = 3). (B) IHC to detect cytokeratin 6 (K6) in Scrib +/+, Scrib +/ fl and Scrib fl/fl adult dorsal epidermis (scale bar = 50 μm, n = 3). (C) IF to detect cytokeratin 5 (K5, green), cytokeratin 10 (K10, red) and DAPI (blue) in Scrib+/+, Scrib +/ fl and Scrib fl/fl adult dorsal epidermis (scale bar = 50 μm, n = 3). Adult dorsal epidermis was harvested from mice 100 days old. (PPTX 755 kb)