12943_2017_679_MOESM2_ESM.tif (740.53 kB)

Additional file 2: Figure S2. of The long non-coding RNA BC200 (BCYRN1) is critical for cancer cell survival and proliferation

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posted on 26.06.2017 by Evan Booy, Ewan McRae, Amit Koul, Francis Lin, Sean McKenna
Optimization of BC200 knock-down by siRNA and LNA GapmeR transfection. (a) HEK293T cells were transfected with the indicated siRNAs and GapmeRs and BC200 expression was assessed 48-h post transfection by RT-qPCR. siRNA_6 and GapmeR_2 were employed for all experiments unless otherwise indicated. (b) GapmeR_2 and GapmeR_3 were transfected into seven different cell lines to test knock-down efficiency. Efficiency of knock-down by GapmeR_3 was greatly reduced in several cell lines tested. (TIFF 740 kb)


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