Additional file 2: Figure S2. of TSG101, a tumor susceptibility gene, bidirectionally modulates cell invasion through regulating MMP-9 mRNA expression

TSG101 depletion does not affect MMP-9 mRNA stability in either HT1080 or HeLaS3 cells. Stability of MMP-9 mRNA in TSG101-depleted HT1080 cells (A and B) and TSG101-depleted HeLaS3 cells (C and D). Cells transfected with control or TSG101 (TSG#1) siRNA were incubated in fresh serum-free medium containing 1 μg/ml actinomycin D for various time points and RT-PCR was performed to monitor turnover of MMP-9 mRNA. Expression levels of MMP-9 (A and C) and 18S rRNA (B and D) mRNAs were analyzed by RT-PCR. Results are plotted as the percentage of mRNA remaining relative to the starting amounts at 0 h individually in control and TSG101 (TSG#1) siRNA transfected cells. The results shown are the means ± S.D. of three independent experiments. Ns, not significant, by Student’s t-test. (PDF 351 kb)