Additional file 2: Figure S2. of Reprogramming of blood cells into induced pluripotent stem cells as a new cell source for cartilage repair

Characterization of hMSCs and hMSC-chondrogenic pellets. (a) Flow cytometric analysis of the hematopoietic markers (CD34, CD45) and MSC markers (CD73, CD105) in hMSCs. The proportion of CD73-expressing cells was 99.7 ± 0.63 %; The proportion of CD105-expressing cells was 98.7 ± 1.21 %. Values represent means ± SEM; n = 3. (b) Three-dimensional pellet culture of the hMSC-chondrogenic pellet. (c) Alcian blue staining and (d) toluidine blue staining of glycosaminoglycans and proteoglycans revealed the chondrocyte-type appearance in the hMSC-chondrogenic pellets. Scale bar = 100 μm. (e,f) Immunohistochemistry for type II and type X collagen. Scale bar = 100 μm. (TIF 8130 kb)