Additional file 2: Figure S2. of Protein deubiquitinase USP7 is required for osteogenic differentiation of human adipose-derived stem cells

The effects of HBX 41,108 on the apoptosis and proliferation of hASCs. (A) hASCs were treated with proliferation or osteogenic media in the presence of vehicle or HBX 41,108. Apoptosis was evaluated by AnnexinV-FITC apoptosis detection kit. Dot plots are representative of two similar experiments. Positive control: cell suspension boiled at 60 °C for 5 min. (B) Growth curves of hASCs cultured in different concentrations of HBX 41,108. Results are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01. hASC human adipose-derived stem cell, OM osteogenic media, PM proliferation media. (PDF 127 kb)