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Additional file 2: Figure S2. of Identification of a potent small molecule capable of regulating polyploidization, megakaryocyte maturation, and platelet production
figure
posted on 2016-12-08, 05:00 authored by Nick Huang, Mabel Lou, Hua Liu, Cecilia Avila, Yupo Ma616452 and TPO independence and synergy. BM and CB CD34+ cells were cultured in the presence of SCF and TPO. In 4Â days of culture, the cells were all healthy and relatively the same size (a,b). By 8Â days of culture, BM cells appeared to have a few (<1%) megakaryocytes with a size approximately four times that of a regular HSC (c). CB however, had no such cells (d). Those cells induced with 616452 for both BM and CB developed a significant number of large MKs (e,f). When CB CD34+ cells were induced with 616452 for 8Â days under various cytokine conditions (g), it was found that 616452 works independently of either SCF or TPO inducing MK maturation under all conditions. (JPG 193 kb)