Additional file 2: Figure S2. of E2-EPF UCP regulates stability and functions of missense mutant pVHL via ubiquitin mediated proteolysis

Missense mutant pVHL constitute E3 ubiquitin complex in cell. (A) HEK293T cells were transfected with plasmid expressing GST tagged hotspot mutant pVHL and/or Flag tagged HIF-1Îą protein and then detected the interaction using GST-pull down assay. (B) HEK293T cells were transfected with plasmid expressing HA tagged mutant pVHL and/or GST tagged Elongin C protein and then detected the interaction using GST-pull down assay. Interacted mutants VHL proteins were detected by immunoblot as indicated. (TIFF 201 kb)