Additional file 2: Figure S2. of Cyclopamine tartrate, an inhibitor of Hedgehog signaling, strongly interferes with mitochondrial function and suppresses aerobic respiration in lung cancer cells

Drp1 localizes to the mitochondrial fission sites in CycT-treated NSCLC H1299 (A) and A549 (B) cells. NSCLC cells were treated with CycT for 24 h. Cells were incubated with anti-Drp1 antibodies, and then with FITC-conjugated goat anti-rabbit secondary antibody, MitoTracker, as well as DAPI. FITC, MitoTracker and DAPI fluorescent images were captured and are shown here. The scale bar indicates 10 μm. (TIF 15003 kb)