12885_2016_2200_MOESM2_ESM.tif (14.65 MB)
Additional file 2: Figure S2. of Cyclopamine tartrate, an inhibitor of Hedgehog signaling, strongly interferes with mitochondrial function and suppresses aerobic respiration in lung cancer cells
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posted on 2016-02-24, 05:00 authored by Md Alam, Sagar Sohoni, Sarada Kalainayakan, Massoud Garrossian, Li ZhangDrp1 localizes to the mitochondrial fission sites in CycT-treated NSCLC H1299 (A) and A549 (B) cells. NSCLC cells were treated with CycT for 24 h. Cells were incubated with anti-Drp1 antibodies, and then with FITC-conjugated goat anti-rabbit secondary antibody, MitoTracker, as well as DAPI. FITC, MitoTracker and DAPI fluorescent images were captured and are shown here. The scale bar indicates 10 μm. (TIF 15003 kb)