Additional file 2: Figure S2. of Chemotherapeutic agents attenuate CXCL12-mediated migration of colon cancer cells by selecting for CXCR4-negative cells and increasing peptidase CD26

Chemotherapeutic drugs increase CD26 protein expression on live cells but do not alter immunoreactivity against dead cells. HT-29 cells were co-stained with CD26-PE-annexin V-PI and gated to necrotic (A; annexin V+/PI+), apoptotic (B; annexin V+/PI−), or live (c; annexin V−/PI−) populations following treatment. Mean channel fluorescence of CD26-PE ± SE (n = 6). *, P < 0.01 compared to vehicle control by two-way ANOVA. (EPS 833 kb)