12885_2015_1702_MOESM2_ESM.eps (833.15 kB)
Additional file 2: Figure S2. of Chemotherapeutic agents attenuate CXCL12-mediated migration of colon cancer cells by selecting for CXCR4-negative cells and increasing peptidase CD26
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posted on 2015-11-10, 05:00 authored by Murray Cutler, Erica Lowthers, Cynthia Richard, Dagmar Hajducek, Paul Spagnuolo, Jonathan BlayChemotherapeutic drugs increase CD26 protein expression on live cells but do not alter immunoreactivity against dead cells. HT-29 cells were co-stained with CD26-PE-annexin V-PI and gated to necrotic (A; annexin V+/PI+), apoptotic (B; annexin V+/PI−), or live (c; annexin V−/PI−) populations following treatment. Mean channel fluorescence of CD26-PE ± SE (n = 6). *, P < 0.01 compared to vehicle control by two-way ANOVA. (EPS 833 kb)