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Additional file 2: Figure S2. of Autophagy-independent function of Atg1 for apoptosis-induced compensatory proliferation

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posted on 2016-08-19, 05:00 authored by Mingli Li, Jillian Lindblad, Ernesto Perez, Andreas Bergmann, Yun Fan
Expression of dAtg1 enhances caspase activity and apoptosis. Late third instar larval eye discs labeled with the cleaved Caspase-3 antibodies (cCasp3, green in A, B, grey in A’, B’, blue in C, and grey in C’), anterior is to the left. (A–B’) Compared to ey > hid-p35 discs (A, A’), cCasp3 labeling indicating activity of Dronc is not affected by expression of dAtg1 which enhances ey > hid-p35-induced overgrowth phenotype (B, B’). (C–C”’) Expression of dAtg1 under control of DE-Gal4 and tub-Gal80ts (DEts) and indicated by GFP. Expression of dAtg1 by a temperature shift (ts) to 29 °C for 48 h induces apoptosis as indicated by cCasp3 labeling (C’, arrow) and developmental defects in the eye disc indicated by the affected pattern of ELAV labeling (C”, arrow). (TIF 4775 kb)

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