Additional file 2: Figure S1. of Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method

Human iPSC line hiPSC1 cultured on LN-521 in E8 medium maintained pluripotent characteristics. A) Typical undifferentiated morphology and B) expression of pluripotency markers NANOG, OCT-3/4, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81, as well as corresponding nuclear stains with DAPI after 4 passages in feeder-free culture. C) Pluripotency shown as expression of markers of the three embryonic germ layers, namely SOX17 for endoderm, OTX2 for ectoderm, and SMA for mesoderm after spontaneous differentiation (feeder-free passage level 4). All scale bars = 200 μm. D) Cells showing normal female karyotype after nine passages. (TIF 5463 kb)