Additional file 2: Figure S1. of SPEN, a new player in primary cilia formation and cell migration in breast cancer

MCF10A cells grown with or without cholera toxin display important phenotypic and functional differences. (a and b) Representative pictures of MCF10A cells grown in the absence (a) or presence (b) of cholera toxin. (c and d) Representative images of primary cilia in MCF10A cells grown in the absence (c) or presence (d) of cholera toxin. Arrows point to primary cilia (scale bar = 5 μm). (e) Cell cycle analyses performed with MCF10A cells grown in the absence and presence of cholera toxin. Bar graph represents the mean percentage of cells (±SEM) in each phase of the cell cycle in three independent experiments. (f) Growth curve of MCF10A cells grown in the presence or absence of cholera toxin. Data points represent the mean fluorescence values (±SEM) of three experiments performed in quadruplicates. (PDF 9130 kb)